@article{Jana_2024, title={Amino acid profile, Antioxidant Activity and flavonoid Content of White Lotus ‘Purain’ from North Bihar, India}, volume={8}, url={http://dx.doi.org/10.22161/ijhaf.8.3.3}, DOI={10.22161/ijhaf.8.3.3}, abstractNote={<jats:p>India’s national flower is the lotus. A lot of seed is carried by white lotuses. Protein and other bioactive substances are abundant in white lotus seeds. An experiment was carried out at the ICAR-National Research Centre in Makhana in 2023-24 to assess the nutritional quality and anti-oxidant characteristics of white lotus seed, which is abundant during the rainy and autumn seasons in the wetland ecosystem of north Bihar. Seeds of white purain accession were grinded and its flour chemically analyzed using AOAC standard methods. Estimation of amino acids was based on the reaction of free amino acids with phenyl isothiocyanate produces stable derivatives that are then separated using liquid chromatography. Anti-oxidant activities were estimated in spectrophotometric observation by DPPH and FRAP methods, based on electron transfer reactions, which visually results on the reduction of a coloured oxidant. Flavonoids are estimated by UHPLC methods. The white lotus purain seed content 17.8±054 % protein, 1.09±0.07 % fat and 1.94±0.08 % crude fibre. The nutritional study also revealed that the appreciable amount of arginine (12.70 g/kg ) and histidine (3.62 g/kg ) were present in the protein of the dry endosperm of Nelumbium spp. In addition, the lotus seed endosperm has a fair source of quercetin (422.0µg/kg) and kaempferol (200.0 µg/kg) as pure flavonoids which influence the rapid glucose metabolism in human cell. The greatest DPPH and FRAP values were found in seed endosperm of white lotus purain, which had 988.05±2.25 mg AEAC/100g FW and 461.78±1.79 mg AEAC/100g FW, respectively.</jats:p>}, number={3}, journal={International Journal of Horticulture, Agriculture and Food science}, publisher={AI Publications}, author={Jana, B. R.}, year={2024}, pages={21–26} }